The Super Eagles midfielder Michael Babatunde appeared to break his arm after being struck by the ball during the 3-2 Group F defeat against Argentina.
Babatunde, 21, was hit by a shot from teammate Ogenyi Onazi during the second half as he tried to move out of the way.
The Volyn Lutsk player looked in distress and was soon stretchered off after being given a protective cast for his arm. Michael Uchebo was substituted on in his place.
Babatunde departed the field of play with Nigeria trailing 3-2, and the scores remained the same at full-time.
Despite the defeat, the Super Eagles advanced out of Group F to the round of 16, with Argentina going through as group winners
Babatunde has endured a frustrating World Cup, having also been stretchered off in the 1-0 win against Bosnia-Herzegovina after picking up an injury.